Figure 1
From: PKIS deep dive yields a chemical starting point for dark kinases and a cell active BRSK2 inhibitor
GW296115 potently inhibits kinases with minimal effect on cell growth. (A) Structure of GW296115. (B) GW296115 does not affect cell growth in multiple cell lines. Cell lines in the panel were treated with 1 μM GW296115 for 72 h (N = 2) and analyzed using the deep dye drop method. MCF10A and MRC-5 are non-malignant cell lines. (C) DiscoverX screen identifies 25 kinases with > 90% inhibition and 41 kinases with ≥ 75% inhibition at 1 μM of GW296115. Coral was used to make (C): https://phanstiel-lab.med.unc.edu/CORAL.
