Figure 4
From: PKIS deep dive yields a chemical starting point for dark kinases and a cell active BRSK2 inhibitor
GW296115 dose-dependently inhibits BRSK2-induced phosphorylation. (A) BRSK2 overexpression induced pULK1 S317, pP62 S351, and pS/T AMPK substrates are decreased dose-dependently by GW296115. HEK293T cells were transiently transfected with hcRED or BRSK2 for 24 h before GW296115 treatment for 2 h. Unformatted images of blots are included in Fig. S2. (B,C) Western blot quantitation for pULK1 s317 and pP62 S351 treated with DMSO or GW296115 at 3.4 μM shows statistically significant changes (N = 3).
