Figure 1

Results of the control experiments. (a,c) rACSF was perfused for 60 min. The 1-min mean for each item was calculated at the 20th and 60th min. The value at the 60th min relative to that at the 20th min was expressed as a ratio. No statistically significant difference was found by the paired t test. preBötC: amplitude, p = 0.083; duration, p = 0.36; and frequency, p = 0.56; XII: amplitude, p = 0.89; duration, p = 0.078; and frequency, p = 0.92). (a) Graph for preBötC, (c) graph for XII. (b,d) rACSF was perfused for 80 min and the 1-min mean for each item was calculated at the 20th and 80th min. The value at the 80th min relative to that at the 20th min was expressed as a ratio. No statistically significant difference was found by the paired t test. (preBötC: amplitude, p = 0.095; duration, p = 0.25; and frequency, p = 0.78; XII: amplitude, p = 0.47; duration, p = 0.056; and frequency, p = 0.24). (b) Graph for preBötC, (d) graph for XII. preBötC pre-Bötzinger complex, XII hypoglossal nucleus.