Figure 4

Increased Ty1 retromobility in rnh1∆ rad27∆ and rnh1∆ rnh201∆ is reversed upon Rnh1 overexpression. (a) Confirmation of Rnh1 over-expression by RT-qPCR. Results are normalized to ACT1 and are presented relative to each mutant’s empty vector control. Statistical significance was assessed by Student’s t-test. Mean ± SD; n = 3. (b) ChIP assessing the effect of Rnh1 over-expression on RNA-cDNA hybrids at Ty1 followed by qPCR directed at TYA (left) and TYB (right). Results are calculated as % input normalized to CUP1 and presented relative to wild-type. Statistical significance was determined using a one-way ANOVA followed by Sidak’s post-hoc test. Mean ± SD; n = 3. (c) Effect of Rnh1 over-expression on Ty1his3AI retromobility. Statistical significance was assessed by a one-way ANOVA followed by Sidak’s post-hoc test. Mean ± SD; n = 5. (a-c) *p < 0.05; **p < 0.01; ****p < 0.0001.