Table 1 Details of primers used in this study.
Genes | Primer sequence forward/reverse (5′–3′) | Tm (°C) | Length (bp) | Efficiency (%) | R2 |
|---|---|---|---|---|---|
ACT | TCGTGTTGCCCCTGAAGAACACCCCGT | 68.8 | 129 | 107.14 | 0.9911 |
/TGGATGGCGACGTACATAGCGGGCA | 67.3 | ||||
GAPDH | ACCCTGAGGAGATCCCATGGGGTGA | 66.34 | 107 | 97.45 | 0.9998 |
/TGCACCACCCTTCAAGTGAGCAGCA | 65.79 | ||||
TUA | ACCAGTGCCTCCACCAACAGCA | 64.55 | 145 | 101.6 | 0.9944 |
/TGCCGCCAATAACTTTGCCAGAGGA | 63.48 | ||||
TUB | AGCTCAGGAACAGTGAGGGCCCTGT | 67.05 | 155 | 101.89 | 0.9762 |
/TGCTGCCTTCGATTCCCTGGTCA | 63.64 | ||||
CYP | TGGGCCAAATACGAACGGGTCCCA | 65.82 | 162 | 105.56 | 0.9975 |
/ACAACAGGCTGCGAAGTCCTCCCA | 65.67 | ||||
UBC | ACCCACCAACTTCATGCAGTGCAGGT | 65.47 | 106 | 98.72 | 0.9815 |
/ACGAAAACACCTCCGGCGTAGGGGCTA | 68.2 | ||||
His | AGGAGGCGTCGAGATTGGCGAGGTA | 66.5 | 123 | 93.12 | 0.9977 |
/TGGTCCCCTCAGAAACAGCGTGCT | 65.67 | ||||
PP2A | TGGACAGGCAGATCGCGCACTT | 64.55 | 127 | 109.07 | 0.9861 |
/ACCGGACACTTGACTGGCTGCACGT | 67.77 | ||||
HSP | TTGTCTGCCTCGGCTCTCTTCCGCA | 67.25 | 177 | 102.4 | 0.9902 |
/TCCTTGCTGCTTGGTGACCGGA | 64.23 | ||||
EF1-α | ACGCTCACGCTCGGCCTTAAGCTT | 66.03 | 176 | 97.9 | 0.9855 |
/TGGTCATTGGCCACGTCGACTCTGG | 65.99 | ||||
NtCER7 | ACCCGACTCACCACGAGGAAGCTGT | 66.87 | 149 | 96.66 | 0.9885 |
/GGTCCTTGACGCAAGCCGCAAGCAT | 67.41 |
