Table 3 Expression stability of the 10 candidate reference genes calculated by BestKeeper analysis.
Rank | Salt treatment | Drought treatment | Heat treatment | Cold treatment | ABA treatment | Different organs | Total samples | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene | CV ± SD | Gene | CV ± SD | Gene | CV ± SD | Gene | CV ± SD | Gene | CV ± SD | Gene | CV ± SD | Gene | CV ± SD | |
1 | ACT | 1.46 ± 0.38 | TUA | 2.10 ± 0.56 | UBC | 2.6 ± 0.74 | TUB | 3.18 ± 0.84 | PP2A | 2.48 ± 0.67 | CYP | 2.38 ± 0.70 | PP2A | 3.52 ± 0.98 |
2 | PP2A | 2.42 ± 0.65 | ACT | 2.63 ± 0.70 | CYP | 2.66 ± 0.77 | TUA | 3.44 ± 0.97 | TUB | 3.05 ± 0.75 | TUB | 2.52 ± 0.68 | ACT | 3.62 ± 0.96 |
3 | GAPDH | 4.41 ± 1.08 | TUB | 2.75 ± 0.78 | TUB | 2.72 ± 0.71 | ACT | 3.66 ± 1.02 | EF1-α | 3.13 ± 0.68 | PP2A | 2.53 ± 0.73 | TUB | 3.84 ± 1.00 |
4 | TUA | 4.23 ± 1.11 | PP2A | 2.94 ± 0.78 | PP2A | 2.75 ± 0.78 | PP2A | 3.62 ± 1.04 | His | 3.43 ± 0.81 | ACT | 2.61 ± 0.72 | CYP | 4.19 ± 1.20 |
5 | TUB | 4.34 ± 1.12 | EF1-α | 3.48 ± 0.82 | ACT | 3.17 ± 0.85 | CYP | 3.77 ± 1.11 | CYP | 3.49 ± 0.95 | TUA | 3.32 ± 0.94 | UBC | 4.48 ± 1.28 |
6 | UBC | 4.28 ± 1.23 | UBC | 3.37 ± 0.97 | GAPDH | 3.32 ± 0.83 | His | 5.67 ± 1.41 | ACT | 3.54 ± 0.90 | EF1-α | 4.42 ± 1.08 | TUA | 4.56 ± 1.21 |
7 | CYP | 4.49 ± 1.30 | GAPDH | 3.53 ± 0.87 | His | 3.67 ± 0.95 | UBC | 5.58 ± 1.57 | GAPDH | 4.15 ± 0.97 | GAPDH | 4.46 ± 1.15 | GAPDH | 4.84 ± 1.19 |
8 | His | 5.26 ± 1.31 | His | 4.74 ± 1.19 | TUA | 3.71 ± 0.96 | GAPDH | 5.71 ± 1.44 | UBC | 3.8 ± 1.07 | His | 4.81 ± 1.27 | EF1-α | 5.27 ± 1.24 |
9 | EF1-α | 5.73 ± 1.36 | CYP | 4.96 ± 1.41 | EF1-α | 4.26 ± 1.01 | EF1-α | 5.78 ± 1.38 | TUA | 4.02 ± 1.01 | HSP | 5.74 ± 1.62 | His | 5.57 ± 1.39 |
10 | HSP | 6.50 ± 1.88 | HSP | 5.91 ± 1.67 | HSP | 9.99 ± 2.50 | HSP | 5.74 ± 1.60 | HSP | 8.29 ± 2.21 | UBC | 5.77 ± 1.73 | HSP | 7.92 ± 2.18 |
