Figure 4 | Scientific Reports

Figure 4

From: Mutational analysis of Escherichia coli GreA protein reveals new functional activity independent of antipause and lethal when overexpressed

Figure 4

fliC::lacZ expression in the presence of weakly induced GreA variants in the greA + ΔdksA host cells. PRG17 (ΔdksA fliC::lacZ distal) strains carrying the plasmid pTrc99a (pControl) or the indicated Class I and III pTrc-GreAMut alleles were grown in LB with or without induction by 0.0125 mM IPTG. Cultures were grown at 37 °C in LB and assayed for β-galactosidase activity at OD600nm of 1.5 and their activities were normalized to activities of uninduced cells. Error bars represent SD from 2 biological replicates and 3 technical replicates. The Class of each mutant is indicated below.

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