Table 2 Bacterial strains and plasmids used in this study.

Strains

MG1655

F-, ilvG, rph1

⁵⁵

GM2163

F-, λ- araC14 leuB6 fhuA31 lacY1 tsx-78 glnX44 galK2 galT22 hisG4 rpsL136(str-r) xylA5 mtl-1 thiE1 dam-13::Tn9 dcm-6 hsdR2 mcrA- mcrB-

Cashel’s lab collection

TE8114

MG1655 dksA::Tc^R

¹⁵

AAG101

MG1655 dksA::Tc greA::Cm

¹⁴

AAG93

MG1655 ∆relA ∆spoT (ppGpp⁰)

⁵⁶

CF11663

MG1655 greB::Km

¹⁴

CF14758

MG1655 ΔgreA ΔgreB

¹⁹

AAG1

MG1655 ∆lacZ

⁵⁷

PRG16

AAG1 fliC::lacZ (+ 1210)

¹⁴

PRG17

AAG1 fliC::lacZ (+ 1210) dksA::Tc^R

¹⁴

PRG18

AAG1 fliC::lacZ (+ 1210) dksA::Tc^R greA::Cm^R

¹⁴

CAG68251

BW25113 rpoBC::Cm rpoB (ΔSI1)

³⁷

LFC1250

MG1655 rpoBC::Cm rpoB (ΔSI1) (P1 transduction)

This study

Plasmids

pTrc99a

colE1 origin, lacI^q, Ptrc expression vector, Ap^R

⁵⁸

pDNL278

lacI^q, greA under control of Ptrc on pTrc99a

³⁹

pHM1701

lacI^q, greA D41A E44Y under control of Ptrc on pTrc99a, Ap^R

Cashel’s lab collection

pHM1885

lacI^q, greA-NTD (stop codon inserted after codon 75) under control of Ptrc on pTrc99a, Ap^R

Cashel’s lab collection

pHM1887

lacI^q, greA-CTD (residues 4–75 deleted) under control of Ptrc on pTrc99a, Ap^R

Cashel’s lab collection

pHM1883

pGB2, pSC101 origin, Ptrc expression vector, Spec^R

¹⁹

pHM1873

pSC101 origin, greA under control of Ptrc on pGB2, Spec^R

¹⁹

pHM1854

pSC101 origin, greA D41A E44Y under control of Ptrc on pGB2, Spec^R

¹⁹

pBbA2k

p15A origin, RFP under a Ptet promoter, Km^R

⁵⁹

pBb-greA-His

greA-his under Ptet promoter on pBbA2k, Km^R

This study