Figure 1 | Scientific Reports

Figure 1

From: Bacteria-induced expression of the pig-derived protegrin-1 transgene specifically in the respiratory tract of mice enhances resistance to airway bacterial infection

Figure 1

Generation of PG-1 transgenic mice. (A) Map of the constructed pTAP-PG-1 plasmid. The piggyBac 5′ and 3′ TRs, the piggyBac transposon 5′ and 3′ terminal repeat elements; TAP, the bovine trachea antimicrobial peptide gene promoter; PG-1, the pig-derived antimicrobial peptide protegrin-1 gene; BGH polyA, bovine growth hormone signal; CMV, cytomegalovirus promoter; Neo, the neomycin gene; 2A, the 2A peptide linker; EGFP, the enhanced green fluorescence protein gene. (B) PCR identification of PG-1 transgenic founder mice. M, marker; P, positive control using mixture of the pTAP-PG-1 plasmid and WT mouse DNA as template for PCR; WT, wild-type mice; N, negative control using water as template for PCR. β-Actin was amplified as internal control. (C) EGFP expression in the claw tissues of PG-1 transgenic founder mice. TG, transgenic mice. WT, wild-type mice.

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