Figure 2

Localization of L-whirlin in the cochlear hair bundles of wild-type mice and in Pcdh15^av-3J/av-3J, Cdh23^−/−, and Adgrv1^−/− mutant mice. (A) The L-whirlin immunolabeling of the ankle-link region of stereocilia in the wild-type mouse is not detected in the Pcdh15^av-3J/av-3J, Cdh23^−/−, and Adgrv1^−/− mice on P6 (white arrows), whereas the apical labeling persists in the mutant mice (arrowheads). Scale bar: 2 µm. (B) Confocal images of hair cells immunostained for either ADGRV1 (above) or usherin (below) (green), and stained for actin (red), in wild-type and Pcdh15^av-3J/av-3J P6 mice. The ADGRV1 and usherin labelings of the ankle-links region are detected both in the wild-type mouse and in the Pcdh15^av-3J/av-3J mutant mouse. Scale bar: 2 µm.