Figure 5

ToxAll elicits B07 epitope (GRA720–28, LPQFATAAT) specific immune response in HLA-B*07:02 mice. (a,b) show IFN-γ -producing T cells from splenocytes of HLA-B*07:02 mice immunized with ToxAll plus GLA-SE compared to Empty plus GLA-SE adjuvant. Data show the specificity of splenocytes from HLA-B*07:02 immunized mice with adjuvanted ToxAll to B07 epitope and not to the pool of HLA-A*02:01 restricted CD8⁺ peptides. The absence of any cross reactivity of HLA haplotypes demonstrates the specificity of that peptide HLA interaction and absence of any contribution of the heterologous combination. (c,d) Luciferase expression from brains of HLA-B*07:02 mice was significantly reduced in ToxAll immunized mice compared to control Empty-SAPN. (d) This reduction in luminescence for the ToxAll SAPN immunized transgenic mice correlates well with the reduction of number of cysts per brain counted shown in e (p = 1.0 because of one outlier in the control group with a much higher number of cysts but the trend show substantial reduction in cyst numbers for all mice by immunization with the ToxAll SAPN administered with GLA-SE).