Figure 7

TEM images. After 24 h co-culture of bone marrow- derived dendritic cells (BMDDCs) from HLA-A*02:01 transgenic mice incubated with (a) GLA-SE alone, (b) ToxAll alone or (c) ToxAll with GLA-SE. GLA-SE present in particles mostly close to cell membranes with a size of ~ 100 nm (blue arrows). When incubated with ToxAll alone, lysosome of BMDDCs didn’t show any processing at 24 h. ToxAll plus GLA-SE interacts with lysosomal enzymes and shows a remarkably homogeneous distribution of particles in the lysosome. The red arrows indicate clusters of gold-encapsulated SAPN.