Figure 7

VEGF knockdown promoted radiosensitivity in nude mouse model. (A) Tumor size was measured at 27 days after subcutaneous inoculation of NC and shRNA cells in nude mice, and the growth curve of tumor nodules with or without 8 Gy irradiation in each group (black arrow indicates the time point of radiation). (B) Expression of LC3 II, p62 and VEGF was analyzed by IHC assay. (C) Tumor nodules were examined by TUNEL assay. (D) Quantitative analysis of TUNEL assay results. **p < 0.01 and ***p < 0.001, size bars = 100 μm.