Figure 1
From: Non-muscle invasive bladder cancer tissues have increased base excision repair capacity
Uracil-initiated total BER activity in NMIBC and their corresponding normal tissues. (a) Representative gel for uracil-initiated total BER activity showing products of 32P-dCTP incorporation (26mer) and ligation (51mer). The lower band represents 1-nt incorporation product (26mer) and the upper band represents the 51mer ligated product. N, the corresponding normal tissue. Quantitation of incorporation (b), ligation (c) and incorporation plus ligation (d) activities from low-grade, high-grade, and Ta/T1 stage NMIBC and their corresponding normal tissues. The incorporation, ligation and incorporation plus ligation activities are presented as PhosphorImager units per min per μg protein. Low-grade sample numbers are 2, 4, 9–12; high-grade sample numbers are 1, 3, 5–8, 13–17. The full-length gels are presented in Supplementary Fig. 1. Gels were visualized by Typhoon FLA 9500 PhosphorImager and analyzed using the ImageQuant software TL v8.1 (https://www.cytivalifesciences.com/en/us/shop/protein-analysis/molecular-imaging-for-proteins/imaging-software/imagequant-tl-8-1-p-00110).
