Figure 2
From: Non-muscle invasive bladder cancer tissues have increased base excision repair capacity
Uracil incision activities and UDG protein levels in NMIBC and their corresponding normal tissues. (a) Representative gel for uracil incision. Incision products are 25mer derived from cleaved 32P-labeled 51mer DNA substrate containing uracil at position 26. N, the corresponding normal tissue. Quantitation of uracil incision from high-grade (b), low-grade (c) and Ta/T1 stages (d) of all NMIBC and their corresponding normal tissues (fmol/μg/min). (e) Western blot analysis of UDG and β-actin in NMIBC and their corresponding normal tissues. Low-grade sample numbers are 2, 4, 9–12; high-grade sample numbers are 1, 3, 5–8, 13–17. (f) Representative gel for single-stranded uracil incision. (g) Quantitation of single-stranded uracil incision. The full-length gels/blots are presented in Supplementary Fig. 2. Radioactive gels were visualized by Typhoon FLA 9500 PhosphorImager and analyzed using the ImageQuant software TL v8.1 (https://www.cytivalifesciences.com/en/us/shop/protein-analysis/molecular-imaging-for-proteins/imaging-software/imagequant-tl-8-1-p-00110).
