Figure 4
From: Non-muscle invasive bladder cancer tissues have increased base excision repair capacity
APE1 activity and protein levels in NMIBC and the corresponding normal tissues. (a) Representative gel for THF incision in NMIBC and their corresponding normal tissues. Incision products are 25mer derived from cleaved 32P-labeled 51mer DNA substrate containing uracil THF at position 26. N, the corresponding normal tissue. Quantitation of THF incision from high-grade (b), low-grade (c) and Ta/T1 stages (d) of all NMIBC and their corresponding normal tissues (fmol/μg/min). (e) Western blot analysis of APE1 and β-actin in NMIBC and their corresponding normal tissues. The full-length gels/blots are presented in Supplementary Fig. 4. Radioactive gels were visualized by Typhoon FLA 9500 PhosphorImager and analyzed using the ImageQuant software TL v8.1 (https://www.cytivalifesciences.com/en/us/shop/protein-analysis/molecular-imaging-for-proteins/imaging-software/imagequant-tl-8-1-p-00110).
