Figure 5
From: Non-muscle invasive bladder cancer tissues have increased base excision repair capacity
Single nucleotide gap-filling activity and POLβ protein levels in NMIBC and their corresponding normal tissues. (a) Representative gel for single nucleotide gap-filling activity showing products of 32P-dCTP incorporation (N, the corresponding normal tissue). Single nucleotide gap-filling activity values of high-grade (b), low-grade (c) and Ta/T1 stages (d) of all NMIBC and the corresponding normal tissues. (e) Western blot of POLβ and β-actin in NMIBC and their corresponding normal tissue extracts. Low-grade sample numbers are 2, 4, 9–12; high-grade sample numbers are 1, 3, 5–8, 13–17. The full-length gels/blots are presented in Supplementary Fig. 5. Radioactive gels were visualized by Typhoon FLA 9500 PhosphorImager and analyzed using the ImageQuant software TL v8.1 (https://www.cytivalifesciences.com/en/us/shop/protein-analysis/molecular-imaging-for-proteins/imaging-software/imagequant-tl-8-1-p-00110).
