Figure 4
From: Non-specific amplification of human DNA is a major challenge for 16S rRNA gene sequence analysis
Pairwise comparison of matched samples using primers targeting the V1–V2 and V3–V4 regions of the 16S rRNA gene fragment. (A) Sample composition at the family level of paired samples. (B) Average Shannon Diversity comparison between samples amplified using V1–V2 primers (red) and V3–V4 primers (blue). (C) Percentage of total sequencing reads aligning to human genome. In both (B) and (C) statistical testing is performed using Wilcoxon signed-rank test.
