Figure 4
From: Three-photon imaging of synthetic dyes in deep layers of the neocortex
Visual responses of OGB-1 AM labelled neurons in layer 5–6 imaged by three-photon microscopy. (a) Anatomical image at cortical depth 550 µm below the pial surface. (b) Tuning curves of direction and orientation selectivity for six neurons (numbered 1 through 6, indicated by arrows in a). Direction Selectivity Index (DSI) and Orientation Tuning Bandwidth (BW, half-width at half-height) of each neuron are labelled accordingly. Open circles represent mean ∆F/F responses and vertical bars lines represent standard deviation; Blue curves are double Von Mises’ fits to the mean data. (c) Anatomical image at cortical depth 600 µm below the pial surface. (d) Tuning curves of direction and orientation selectivity for six neurons at 600 µm below pia (numbered 1 through 6, indicated by arrows in c).
