Figure 5
From: Iodine staining as a useful probe for distinguishing insulin amyloid polymorphs
Resonance Raman spectra of iodine-stained insulin amyloid fibrils with excitation of 514.5 nm (a,b) and 785 nm (c,d). The spectra of NaCl fibrils (a,c) and SDS fibrils (b,d) are shown. Samples containing 0.5 mg/ml daughter fibrils, 0.6 mM KI, and 0.08 mM I2 in 25 mM HCl were subjected to the measurements. Solid and dotted lines represent spectra of iodine-stained fibrils and unstained fibrils measured as a control, respectively, and the positions of peaks are indicated by black dashed lines.
