Figure 1

PCSK9 levels and hematologic parameters in WT mice and SCD mice, with and without Pcsk9 deficiency. (A) Concentrations of circulating PCSK9 in Wt, Wt^bmt, Pcsk9^+/+, SCD^bmt, and Pcsk9^−/−, SCD^bmt mice (n = 5, each group). Circulating blood count data for (B) erythrocyte number (C) hemoglobin (D) hematocrit (n = 5, 14, and 12 for Wt, Wt^bmt, Pcsk9^+/+, SCD^bmt, and Pcsk9^−/−, SCD^bmt mice, respectively). (E) Quantification of circulating reticulocytes by new methylene blue staining (n = 9, each group). (F) Percent population of whole blood which stained dually with Ter119-APC-Cy7 and Annexin V-PE. (n = 9, each group) (G) Percentage of erythrocytes that sickled at 90 min post-introduction of 2% sodium metabisulfite; n = 4 and n = 5, for Pcsk9^+/+, SCD^bmt, and Pcsk9^−/−, SCD^bmt mice, respectively. Representative images erythrocytes sickling from (H) Pcsk9^+/+, SCD^bmt and (I) Pcsk9^−/−, SCD^bmt at the 90 min time point. Error bars denote SEM. Asterisks indicate significance to Wt, Wt^bmt: * < 0.05, ** < 0.01, *** < 0.005, **** < 0.001; Crosses indicate significance between Pcsk9^+/+, SCD^bmt and Pcsk9^−/−, SCD^bmt mice using the same scale.