Figure 3

Measurements of other parameters. The following metrics were determined and compared in fundus images from diabetic and non-diabetic mice using our image analysis. (A) Number of Extremities at week 0—WT: 104.2 ± 8.348, Akita/+ : 59 ± 12.93 (P < 0.0001); week 9—WT: 114.7 ± 9.11, Akita/+ : 64.89 ± 12.62 (P < 0.0001); week 16—WT: 145.2 ± 25.95, Akita/+ : 88.11 ± 24.87 (P = 0.0002); and at week 24—WT: 143 ± 27.04, Akita/+ : 140.7 ± 22.95 (P = 0.846). (B) Number of Nodes at week 0—WT: 78.44 ± 12.17, Akita/+ 33.44 ± 11.16 (P < 0.0001); week 8—WT: 93.33 ± 17.74, Akita/+ : 30 ± 12.29 (P < 0.0001); week 16—WT: 132.7 ± 33.4, Akita/+ : 59.22 ± 23.17 (P < 0.0001); and week 24—WT: 136 ± 35.02, Akita/+ : 91.56 ± 17.5 (P = 0.0035). (C) Number of Junctions at week 0—WT: 22.89 ± 2.892, Akita/+ : 25.56 ± 11.11 (P = 0.0496); week 8—WT: 22.56 ± 3.644, Akita/+ : 13.67 ± 3.606 (P < 0.0001); week 16—WT: 23.33 ± 4.77, Akita/+ : 31 ± 9.912 (P = 0.0528); and week 24—WT: 35.33 ± 7.583, Akita/+ : 57.11 ± 16.14 (P = 0.0021). (D) Number of Branches at week 0—WT: 27.78 ± 1.302, Akita/+ : 19.78 ± 5.357 (P = 0.0005); week 8—WT: 31.33 ± 4.416, Akita/+ : 20 ± 6.205 (P = 0.0004); week 16—WT: 45.33 ± 15.87, Akita/+ : 62.44 ± 12.24, P = 0.0209; and week 24—WT: 51.89 ± 14.47, Akita/+ : 67.22 ± 16.73 (P = 0.054). (E) Total Branch Length at week 0—WT: 1905 ± 302.5, Akita/+ : 1653 ± 543.5 (P = 0.2416); week 8—WT: 2203 ± 554.6, Akita/+ : 1701 ± 299.3 (P = 0.0296); week 16—WT: 3521 ± 722.7, Akita/+ 2784 ± 1076 (P = 0.1073); and week 24—WT: 7390 ± 1506, Akita/+ : 6500 ± 1565 (P = 0.2369). (F) Number of Segments at week 0—WT: 14.33 ± 5.809, Akita/+ : 17.56 ± 3.432 (P = 0.4256); week 8—WT: 18.11 ± 7.132, Akita/+ : 23.89 ± 2.406 (P = 0.1069); week 16—WT: 42.78 ± 11.36, Akita/+ : 43.33 ± 5.302 (P = 0.9331); and week 24—WT: 63.67 ± 18.06, Akita/+ : 58.00 ± 3.775 (P = 0.4369). (G) Total Segment Length at week 0—WT: 1271 ± 280.1, Akita/+ : 925.3 ± 51.22 (P = 0.0022); week 8—WT: 1600 ± 235.2, Akita/+ : 992.3 ± 96.78 (P < 0.0001); week 16—WT: 1792 ± 291.7, Akita/+ : 1421 ± 278.1 (P = 0.0138); and week 24—WT: 2326 ± 857.1, Akita/+ : 2209 ± 367.3 (P = 0.7102). (H) Number of Isolated Segments at week 0—WT: 36.11 ± 6.936, Akita/+ : 17.56 ± 10.3 (P = 0.93); week 8—WT: 49.89 ± 11.27, Akita/+ : 29.11 ± 6.52 (P = 0.0139); week 16—WT: 53 ± 17.2, Akita/+ : 86.89 ± 9.905 (P = 0.0001); and week 24—WT: 59.78 ± 16.77, Akita/+ : 83.11 ± 22.78 (P = 0.0249). (I) Isolated Element Length at week 0—WT: 448 ± 1533, Akita/+ : 2739 ± 837.5 (P = 0.0084); week 8—WT: 5645 ± 1580, Akita/+ : 2835 ± 1264 (P = 0.0007); week 16—WT: 10,958 ± 1554, Akita/+ : 7224 ± 1505 (P < 0.0001); and week 24—WT: 14,234 ± 2725, Akita/+ : 22,362 ± 6383 (P = 0.0029). Initially, the differences in number of extremities, number of nodes, number of branches, total segments length, and isolated elements length were statistically significant. At week 8, the differences in number of extremities, number of nodes, number of junctions, number of branches, total branches length, number of isolated segments, total segments length, and isolated elements length were statistically significant. At week 16, the differences in number of extremities, number of nodes, number of branches, number of isolated segments, total segments length, and isolated elements length were statistically significant. At week 24, the differences in number of nodes, number of junctions, number of isolated segments, and isolated elements length were statistically significant.