Figure 4

Measurements of additional parameters. The following metrics were determined and compared in fundus images from diabetic and non-diabetic mice using our image analysis. (A) Number of Pieces at week 0—WT: 86.44 ± 10.33, Akita/+ : 76.78 ± 12.37 (P = 0.0908); week 8—WT: 90.78 ± 7.12, Akita/+ : 72.33 ± 15.75 (P = 0.0056); week 16—WT: 94.22 ± 25.16, Akita/+ : 91.33 ± 26.61 (P = 0.8049); and week 24—WT: 114 ± 31.16, Akita/+ : 103.8 ± 35.92 (P = 0.5281). (B) Total Length at week 0—WT: 7713 ± 989.9, Akita/+ : 5264 ± 922.5 (P < 0.0001); week 8—WT: 8713 ± 666, Akita/+ 4545 ± 1892 (P < 0.0001); week 16—WT: 13,754 ± 4766, Akita/+ : 19,128 ± 3160 (P = 0.0123); and week 24—WT: 17,419 ± 2862, Akita/+ : 22,654 ± 3205 (P = 0.0021). (C) Total Branching Length at week 0—WT: 3180 ± 373.3, Akita/+ : 3184 ± 110 (P = 0.9908); week 8—WT: 4725 ± 693.7, Akita/+ : 3908 ± 1962 (P = 0.256); week 16—WT: 15,809 ± 4450, Akita/+ : 10,835 ± 1300 (P = 0.0054); and week 24—WT: 22,594 ± 6343, Akita/+ : 18,199 ± 4032 (P = 0.0985). (D) Branching Interval at week 0—WT: 45.74 ± 9.49, Akita/+ : 49.04 ± 9.931 (P = 0.4815); week 8—WT: 51.84 ± 9.847, Akita/+ : 53.77 ± 15.41 (P = 0.7567); week 16—WT: 43.08 ± 12.62, Akita/+ : 23.39 ± 5.421 (P = 0.0005); and week 24—WT: 47.43 ± 22.31, Akita/+ : 34.35 ± 8.573 (P = 0.1202). (E) Number of Master Segments at week 0—WT: 7.889 ± 0.928, Akita/+ : 6.00 ± 0.5774 (P = 0.0108); week 8—WT: 11.33 ± 2.449, Akita/+ : 10.78 ± 2.819 (P = 0.6613); week 16—WT: 11.78 ± 4.265, Akita/+ : 12.56 ± 7.091 (P = 0.7816); and week 24—WT: 14.89 ± 3.06, Akita/+ : 15.01 ± 3.41 (P = 0.9926). (F) Total Master Segment Length at week 0—WT: 1149 ± 60.66, Akita/+ : 899.1 ± 88.25 (P < 0.0001); week 8—WT: 1433 ± 250.7, Akita/+ : 909 ± 161.8 (P < 0.0001); week 16—WT: 2231 ± 488.5, Akita/+ : 1790 ± 533.7 (P = 0.086); and week 24—WT: 3249 ± 942.9, Akita/+ : 1871 ± 472.9 (P = 0.0012). (G) Number of Master Junctions at week 0—WT: 6.667 ± 1.225, Akita/+ : 5.556 ± 2.297 (P = 0.2187); week 8—WT: 9.22 ± 1.641, Akita/+ : 7.667 ± 1.936 (P = 0.0847); week 16—WT: 10.89 ± 1.453, Akita/+ : 12.44 ± 2.92 (P = 0.1717); and week 24—WT: 15.22 ± 4.177, Akita/+ : 14.89 ± 4.649 (P = 0.8749). Initially, the differences in total length, number of master segments, and total master segment length were statistically significant. At week 8, the differences in number of pieces, total length, and total master segment length were statistically significant. At week 16, the differences in total length, total branching length, and branching interval were statistically significant. At week 24, the differences in total length, and total master segment length were statistically significant.