Figure 7

Biological effects of AL3-containing liposomes in neuroblastoma cells. (A) IMR32 cells were treated for 6 days with BMOV or AL3 in solution, empty liposomes, or AL3 liposomes (batch 1; both undialysed and dialysed [D]) after which cell viability was assessed by nuclei counting. ANOVA with Dunnet post hoc using ethanol (etoh) as the control, *p = 0.053 (n = 3). (B) SK-N-SH cells were treated for 5 days with BMOV or AL3 in solution, undialysed empty liposomes, or batch-1 undialysed (AL3 LIPO) and dialysed (AL3 LIPO DIA) AL3 liposomes. Neurite formation (white arrows) is indicated. (C) Neurite lengths were measured after treatments with BMOV, AL3, empty liposomes and AL3 liposomes. Dialysed liposome treatments are indicated as “dia”. Empty liposome dosage in parenthesis matches the lipid content of those used in the equivalent AL3 liposome treatments. ANOVA with Dunnett post hoc using untreated ethanol (etoh) as control **p < 0.01, ***p < 0.001 (n = 3). (D) SK-N-SH cells were treated in the same way as in (B) and (C), using batch 2 and 3 AL3 liposomes, showing batch 1 data again for comparison. Mean average effects on neurite length are shown.