Figure 2
From: A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR
The acid-pH method provides comparable results to commercial kits in clinical samples. Bars represent the mean + /- standard deviation Cq values for each RT-qPCR target gene N1, N2, and RNase P, for samples with Cq N1 ≤ 36 (A) and with Cq N1 > 36 (B). Each dot represents one sample. Orange bars show results obtained with High Pure Viral RNA Kit (Roche). Blue bars show results obtained with the acid pH method. Pairwise comparisons of mean Cq values for each target gene were done using a two-tailed paired Student’s t-test, with a confidence level of 95%. ‘ns’ means no statistically significant differences.
