Figure 1
(a) SANS, synchrotron SAXS and cryo-TEM of native liposomes in absence of MBP. The SANS and SAXS data have been globally fitted with a simple box model. The fits are indicated by continuous lines. (b) SANS and synchrotron SAXS of native liposomes with 16.7 wt. ratio MBP and cryo-TEM of native liposomes with 40 wt. ratio MBP. The lipid concentration of the SANS measurements was 2.5 mg/ml, in synchrotron SAXS 5 mg/ml and in cryo-TEM 1 mg/ml. The concentration in SAXS was chosen higher for intensity reasons and the concentration of cryo-TEM lower as the blotting process increases the intensity again. The black bar in the cryo-TEM images corresponds in each case to 100 nm. In (c) and (d) SAXS data of native and EAE modified liposomes with MBP (9, 16.7, 28.6 and 50 wt. ratio) have been fitted globally by a LUV + MLV model in the region of interest. SAXS data with 0 wt. ratio MBP were fitted with the LUV model. The lipid concentrations in the SAXS measurements without MBP were 10 mg/ml and with MBP 5 mg/ml. Here, the global fit parameters of SAXS and SANS measurements of the respective lipid compositions are used for the LUV part of the model. These fits are indicated by the dashed black lines in the respective plots. For better visibility the different datasets in (c) and (d) have been vertically shifted.
