Figure 3

Kinetics of MBP-mediated assembly process followed by SAXS. SAXS data of (a) native and (b) EAE modified membranes mixed with 16.7 wt. ratio MBP 0, 2, 4, 6 and 8 days before the measurement. In c) and d) cryo-TEM pictures of aged native (5 days) and EAE modified (6 days) liposomes with 16.7 wt. ratio MBP are shown. The black bar in (c) corresponds to 500 nm and in (d) to 100 nm. For better visibility the different datasets in (a) and (b) have been vertically shifted. In c) some of the rounded edges and in d) some of the sharp edges have been marked by red arrows. The samples for SAXS and cryo-TEM were taken from the same batch for better comparison and had a lipid concentration of 5 mg/ml.