Figure 5
From: Sectoral activation of glia in an inducible mouse model of autosomal dominant retinitis pigmentosa
SD-OCT hyper-reflectivity and retinal thinning occur within one hour of exposure to bright white light. Four I307N Rho mice were challenged with 20,000 lx of light for thirty minutes. Serial SD-OCT scans were then obtained at multiple time points that spanned from fifteen minutes after the cessation of light exposure up to eight days thereafter. (a) Shown are representative 140 µm segments of SD-OCT B-scans from either the superotemporal or inferonasal retina at each time point for a single mouse. ONL hyper-reflectivity in the inferonasal retina was visually apparent as early as fifteen minutes and the retina appeared to thin after light treatment. (b) Total retinal thickness (TRT) was measured as the distance from the superficial aspect of the NFL to the deep aspect of the RPE. The TRT was recorded at six predetermined positions relative to the ONH, three in the superotemporal and inferonasal retina each, which were then averaged within and between eyes. (c) Longitudinal reflectivity profiles were obtained with ImageJ to measure reflectivity, which was recorded at the same three positions in the superotemporal and inferotemporal retinas as for the TRT and with the same averaging scheme. Measurements were not performed beyond the day one time point since we previously determined this to be the maximal response7. The bar graphs represent either the mean TRT or mean reflectivity ± s.e.m. (n = 4). For both TRT and reflectivity, two-way repeated-measures ANOVA for matching data (for both time and retinal sector variables) followed by Tukey’s multiple comparisons tests were performed to determine statistical significance. The multiple comparisons tests compared each retinal segment at a given time point to its corresponding baseline (reported as asterisks above each bar graph) and to the opposite side of the retina at the same time point (reported as asterisks above each horizontal line segment). The factors of time and retinal sector, as well as their interaction, were statistically significant. Normality of residuals was assessed with Shapiro–Wilk test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Statistical data can be viewed in Supplementary Table S4 and S5.
