Figure 1 | Scientific Reports

Figure 1

From: Importance of cholesterol-rich microdomains in the regulation of Nox isoforms and redox signaling in human vascular smooth muscle cells

Figure 1

Localization of Nox isoforms and caveolin-1 (Cav-1) in humans small arteries. Paraffin-embedded sections of human small arteries were immunostained with Nox4, Nox5 and Cav-1. (A) Nox5 partially colocalizes with Cav-1 in the vascular media. (B) Nox4 does not collocalize with Cav-1. Green immunofluorescence indicates Nox4 or Nox5 immunostaining, red indicates Cav-1 staining and blue indicates nuclear staining using DAPI. After probing with the respective antibodies, slides were mounted, images were recorded in an Axiovert 200M microscope with a laser scanning module LSM 510 (Carl Zeiss AG, Heidelberg, Germany) using a × 40 objective. Image processing was obtained by Image J. Asterisk: vascular media comprising VSMCs. Arrows: Endothelium. Arrowhead: partial-colocalization of Nox5 and Cav-1 (orange colour). Cav-1 Caveolin-1, IgG immunoglobulin G.

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