Figure 11
ROS generation and vascular signaling in Cav-1-deficient mice. Wild type (WT) and Cav-1−/− mice were infused with vehicle (Veh; saline) or Ang II (400 ng/kg/min) and after two weeks, mesenteric arteries and aortas were isolated for NADPH-dependent ROS generation measurement and protein expression. (A) NADPH-derived O2− generation measured by lucigenin assay in mesenteric arteries. (B) Phosphorylation of Ezrin/Radixin/Moesin and ERK1/2 in aortas isolated from WT and Cav-1−/− mice. β-Actin was used as loading control. Data are means ± SEM from 5–7 experiments. *P < 0.05 vs. WT. Ang II angiotensin II, Cav-1 caveolin-1, Cav-1−/− caveolin-1 deficiency, Veh vehicle, RLU relative light units, p-ERM phosphorylated Ezrin–Radixin–Moesin, p-ERK1/2 phosphorylated ERK1/2.
