Figure 2 | Scientific Reports

Figure 2

From: Importance of cholesterol-rich microdomains in the regulation of Nox isoforms and redox signaling in human vascular smooth muscle cells

Figure 2

Nox1 and Nox5 but not Nox4 are present in lipid-rafts/caveolae in human VSMCs. VSMCs isolated from small arteries were stimulated with Ang II and subjected to discontinuous sucrose density gradient. (A) Representative immunoblots of Nox5 and Cav-1. Nox5 is present in isolated lipid-rafts fractions 3–4 in human VSMCs. (B) Representative images of Nox5 and Nox1 in lipid rafts (LRs) vs. non-lipid rafts (NLRs) in VSMCs stimulated with Ang II (100 nmol/L) for 5, 15 or 30 min. Isolated LR (fractions 3–4) or high density NLR (fractions 7–12) were pooled together and the percentage ratio of LRs vs. NLRs for Nox5 (C) and Nox1 (D) was determined. (E) Representative images of Nox4 in LRs vs. NLRs. Bar graphs are means ± SEM from 4–5 experiments. Control was taken as 100% and data are presented as the percentage changes relative to control conditions. *P < 0.05 vs. Ctl. #P < 0.05 vs. Ang II, 5 min. Ang II angiotensin II, Ctl control, Cav-1 caveolin-1, LR lipid rafts, NLR non-lipid rafts, M marker, THP1 human acute monocytic leukemia cells, VSMCs vascular smooth muscle cells.

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