Figure 3 | Scientific Reports

Figure 3

From: Importance of cholesterol-rich microdomains in the regulation of Nox isoforms and redox signaling in human vascular smooth muscle cells

Figure 3

Differential effects of lipid-raft disruption on Ang II–induced ROS production. VSMCs were treated with methyl-b-cyclodextrin (MCD; 10 mmol/L) for 45 min or Nystatin (Nys; 50 μg/mL) for 30 min and stimulated with Ang II (100 nmol/L) for 5 and 15 min. (A) NADPH-derived O2− generation measured by lucigenin assay in human VSMCs. (B) H2O2 levels were measured by the Amplex Red assay. Data are mean ± SEM from 5–12 experiments. Control was taken as 100% and data are presented as the percentage changes relative to control conditions. *P < 0.05 vs. Ctl. Ang II angiotensin II, Ctl control, H2O2 hydrogen peroxide, RLU relative light units.

Back to article page