Figure 9 | Scientific Reports

Figure 9

From: Importance of cholesterol-rich microdomains in the regulation of Nox isoforms and redox signaling in human vascular smooth muscle cells

Figure 9

DJ1 oxidation—effects of Ang II and lipid raft disruptors. VSMCs treated with Ang II were subjected to discontinuous sucrose density gradient. (A) Representative immunoblots of total and irreversibly oxidised DJ1 in lipid rafts (LRs) vs. non-lipid rafts (NLRs). (B) VSMCs were stimulated with Ang II (100 nmol/L, 5 and 15 min) or treated with lipid-raft disruptors (10 mmol/L MCD or 50 μg/mL Nys). Representative immunoblots and quantification of total and irreversible oxidised DJ1. β-Actin was used as loading control. Data are means ± SEM from 7 experiments. Control was taken as 100% and data are presented as the percentage changes relative to control conditions. *P < 0.05 vs. control. Ang II angiotensin II, Ctl control, Cav-1 caveolin-1, LR lipid raft, NLR non-lipid raft, M marker, MCD methyl-b-cyclodextrin, Nys nystatin.

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