Figure 5 | Scientific Reports

Figure 5

From: Circadian regulation of diel vertical migration (DVM) and metabolism in Antarctic krill Euphausia superba

Figure 5

LD-DD shortening of the oscillation period in the core clock genes clk, cyc and per. (A–C) Line-plots representing changes in expression levels over time for the clock genes clk (A), cyc (B) and per (C) in LD (blue) and DD (red) in the brain, and for the clock gene per (D) in LD (blue) and DD (red) in the eyestalks. Time intervals are reported on the x-axis. For LD, Zeitgeber Time (ZT) is used, given in hours from the time of lights-on (6:00). For DD, Circadian Time (CT) is used, given in hours from the beginning of the subjective light phase (6:00). Mean gene expression levels (n = 7) are reported on the y-axis as mean Relative Quantities (RQ), indicating the average normalized expression levels of the target clock genes relative to the expression levels of the selected internal and external reference genes at each time interval. Error bars represents SEMs (n = 7). Results of the RAIN analysis test for the presence of rhythmic oscillations are reported (T = period of oscillation; p = p-value). A schematic representation of the light/dark cycle is given below the graphs. For LD, withe rectangles indicate light phases, grey rectangles indicate dark phases. For DD, light grey rectangles indicate subjective light phases, grey rectangles indicate dark phases. The figure was generated using the plot function in the “graphics” package (version 3.6.3, https://www.rdocumentation.org/packages/graphics) in R (RStudio version 1.0.136, RStudio Team 2016).

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