Table 2 Top 20 expressed novel miRNAs identified in the liver tissue of Angus, Charolais, and KC steers.
Provisional ID | miRDeep2 score | Estimated probability that the miRNA candidate is a true positive | Total read count | Mature miRNA consensus sequence |
|---|---|---|---|---|
Angus | ||||
bta-miR-AB-10 | 18,895.5 | 83 ± 5% | 37,061 | aaagcugaaugaacuuuuuggc |
bta-miR-AB-9 | 4.9 | 77 ± 4% | 35,497 | agagaugaagcacuggagc |
bta-miR-AB-122 | 5.5 | 83 ± 4% | 9964 | ugggcugcagugcgcuaugcc |
bta-miR-AB-83 | 3438.3 | 83 ± 5% | 6743 | aaaaccugaaugaacuuuu |
bta-miR-AB-93 | 1927.8 | 83 ± 5% | 3784 | aaagaaguuuguuuggguuuu |
bta-miR-AB-59 | 5.1 | 83 ± 4% | 3766 | caaaaaguuuguuuggguuuu |
bta-miR-AB-65 | 1854.7 | 83 ± 5% | 3641 | aaaaagguuuguuuggguuuu |
bta-miR-AB-27 | 1789.4 | 83 ± 5% | 3501 | aaaaaaguuuguuuggauuuu |
bta-miR-AB-95 | 5.2 | 83 ± 4% | 3466 | aaaaaaguuuguguggguuuu |
bta-miR-AB-52 | 1663.5 | 83 ± 5% | 3254 | aaaaaaguuuguuugguuuuu |
bta-miR-AB-29 | 1435.9 | 83 ± 5% | 2816 | acucgaacgaauuuuuggcc |
bta-miR-AB-3 | 4.8 | 77 ± 4% | 2725 | guccaguuuucccaggaa |
bta-miR-AB-2 | 6.2 | 84 ± 5% | 1536 | gggggccggcggcggcggcggc |
bta-miR-AB-54 | 4.6 | 77 ± 4% | 1210 | gaaaaaguuuguuuggguuu |
bta-miR-AB-67 | 4.3 | 77 ± 4% | 1116 | aaaaaaguuuguuugggauu |
bta-miR-AB-28 | 4.8 | 77 ± 4% | 1051 | caaaaaguucguccagauuuu |
bta-miR-AB-12 | 4.9 | 77 ± 4% | 1041 | aucccacuucugacacca |
bta-miR-AB-23 | 502 | 83 ± 5% | 985 | acaaccugaaugaacuuuuuga |
bta-miR-AB-19 | 5.1 | 83 ± 4% | 976 | ucaaguagcucacagucuag |
bta-miR-AB-63 | 467.3 | 83 ± 5% | 915 | ggaauaccggguacuguaggcu |
Charolais | ||||
bta-miR-AB-10 | 29,069.9 | 77 ± 6% | 57,018 | aaagcugaaugaacuuuuuggc |
bta-miR-AB-9 | 4.9 | 70 ± 4% | 54,090 | agagaugaagcacuggagc |
bta-miR-AB-3 | 4.8 | 70 ± 4% | 3570 | guccaguuuucccaggaa |
bta-miR-AB-29 | 1680.6 | 77 ± 6% | 3296 | acucgaacgaauuuuuggcc |
bta-miR-AB-19 | 5.1 | 78 ± 5% | 1897 | ucaaguagcucacagucuag |
bta-miR-AB-2 | 6.2 | 78 ± 5% | 1291 | gggggccggcggcggcggcggc |
bta-miR-AB-12 | 4.9 | 70 ± 4% | 1173 | aucccacuucugacacca |
bta-miR-AB-23 | 569.8 | 77 ± 6% | 1118 | acaaccugaaugaacuuuuuga |
bta-miR-AB-148 | 561.9 | 77 ± 6% | 1101 | uuguccgacucuuagcgg |
bta-miR-AB-28 | 4.8 | 70 ± 4% | 1046 | caaaaaguucguccagauuuu |
bta-miR-AB-137 | 416.7 | 77 ± 6% | 818 | aaaucugaacaagcuuuuuggc |
bta-miR-AB-156 | 406.5 | 77 ± 6% | 796 | aaaaaguucguuuggguuuuu |
bta-miR-AB-7 | 401.1 | 77 ± 6% | 785 | aaaacugaaugaacauuuuggc |
bta-miR-AB-48 | 333.4 | 77 ± 6% | 653 | cgaaaaguucguuuggguuuu |
bta-miR-AB-47 | 251.3 | 77 ± 6% | 491 | aaaaguucguuucgguuuuucc |
bta-miR-AB-145 | 4.3 | 70 ± 4% | 381 | ucuuggagcucaccgucuag |
bta-miR-AB-168 | 4.7 | 70 ± 4% | 375 | cugaccuaugaauugaag |
bta-miR-AB-158 | 190.1 | 77 ± 6% | 372 | aaaaaguuccuuuggguuuuc |
bta-miR-AB-34 | 174.6 | 77 ± 6% | 341 | ucuagaagcucacagucuag |
bta-miR-AB-146 | 171.8 | 77 ± 6% | 335 | uucucagguuggacaguccuga |
Kinsella composite (KC) | ||||
bta-miR-AB-10 | 32,819.1 | 80 ± 5% | 64,372 | aaagcugaaugaacuuuuuggc |
bta-miR-AB-9 | 4.9 | 74 ± 4% | 51,210 | agagaugaagcacuggagc |
bta-miR-AB-225 | 3118.6 | 80 ± 5% | 6111 | cucucgagucgcgacguguaucuc |
bta-miR-AB-59 | 5.1 | 80 ± 4% | 5151 | caaaaaguuuguuuggguuuu |
bta-miR-AB-65 | 2566.5 | 80 ± 5% | 5037 | aaaaagguuuguuuggguuuu |
bta-miR-AB-206 | 5.2 | 80 ± 4% | 5028 | gaaaaaguuuguuuggguuuu |
bta-miR-AB-27 | 5.3 | 80 ± 4% | 4809 | aaaaaaguuuguuuggauuuu |
bta-miR-AB-3 | 4.8 | 74 ± 4% | 4745 | guccaguuuucccaggaa |
bta-miR-AB-52 | 2256.4 | 80 ± 5% | 4417 | aaaaaaguuuguuugguuuuu |
bta-miR-AB-29 | 2249.6 | 80 ± 5% | 4412 | acucgaacgaauuuuuggcc |
bta-miR-AB-63 | 1079.1 | 80 ± 5% | 2115 | ggaauaccggguacuguaggcu |
bta-miR-AB-54 | 4.6 | 74 ± 4% | 1591 | gaaaaaguuuguuuggguuu |
bta-miR-AB-28 | 4.8 | 74 ± 4% | 1502 | caaaaaguucguccagauuuu |
bta-miR-AB-23 | 750.3 | 80 ± 5% | 1472 | acaaccugaaugaacuuuuuga |
bta-miR-AB-67 | 4.3 | 74 ± 4% | 1434 | aaaaaaguuuguuugggauu |
bta-miR-AB-12 | 4.9 | 74 ± 4% | 1429 | aucccacuucugacacca |
bta-miR-AB-2 | 6.2 | 80 ± 5% | 1320 | gggggccggcggcggcggcggc |
bta-miR-AB-18 | 406.1 | 80 ± 5% | 790 | aaacccugaaggaacuuuu |
bta-miR-AB-7 | 376.6 | 80 ± 5% | 737 | aaaacugaaugaacauuuuggc |
bta-miR-AB-198 | 289.8 | 80 ± 5% | 567 | aaaaucugaacaaacuuuu |
