Figure 7
In vivo Persistence of ΔBPSS0899, ΔBPSS1321, and ΔBPSL1494 in the Absence of Treatment. Mice were intranasally infected with 1.5 LD50 of wild type or CFU equivalent of each mutant and (A) survival and (B) weight was monitored for 14 days after which organs of surviving mice were collected, (C) visualized for gross pathology, and CFU burden enumerated. (D) Lungs, (E) livers, and (F) spleens were homogenized and bacteria cultured on agar to assess bacterial burden. Statistics were completed using Two-Way ANOVA or One-Way ANOVA. *P < 0.05 and **P < 0.01.
