Figure 2

Platelets and PBMCs were isolated from Simvastatin users and controls as described in the text, and scatter plots are shown for the following mitochondrial respiratory parameters: basal OCR (A and G), ATP turnover (B and H), reserve respiratory capacity (C and I), maximal respiratory capacity (D and J), ECAR (E and K), and glycolytic reserve (F and L). Values for OCR were normalized to the basal OCR, and ECAR was normalized to control acidification rate. All values are shown ± standard error of the mean. Individual data points correspond to 13 controls and 58 Simvastatin users. An antibody-based dipstick assay was used to quantify active forms of complex I and complex IV subunits in platelets from Simvastatin users and controls (M and N). Individual data points correspond to eleven biological replicates.