Figure 1
From: MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases
Distress analysis during chronic pancreatitis. Pancreatitis was induced by repetitive, i.p. injections of cerulein, three times a week. The distress was assessed before any intervention (day-2), in the early phase (day 2) and at the late phase (day 30) of disease progression (a), by assessing body weight change (b), burrowing behavior (c), nesting-score (d) distress-score (e), FCMs (f). Just before euthanasia (euth.) corticosterone was assessed in the plasma of mice (g). Statistical significance of parametric data was calculated by RM one-way ANOVA, followed by Tukey’s multiple comparisons test (b, c, e). Non-parametric data was analyzed, by Friedman test followed by Dunn’s multiple comparisons test (d, f) or Kruskal–Wallis test, followed by Dunn’s multiple comparison (g). P < 0.05: significant to indicated phase. a–f (n = 8), g control (n = 11), early (n = 9), late (n = 2).
