Figure 3

Distress assessment after bile duct ligation. The bile duct ligation was performed on day 0. MCC950 (treatment) or vehicle solution (experimental) were applied as daily i.p. injection from day-1 to day 13. Distress was assessed before any intervention (day-3) and at the late phase (day 13) of disease progression (a), by assessing body weight change (b), burrowing behavior (c), nesting-score (d) and a distress-score (e) or by assessing just before euthanasia (euth.) plasma corticosterone concentration (f). Significant differences of non-parametric data were calculated by Kruskal–Wallis test and Dunn’s method for multiple comparisons (b–f). P < 0.05 was considered to be significant; b + e: control (n = 24), experimental (n = 12), treatment (n = 12), c + d: control (n = 10), experimental (n = 5), treatment (n = 5), f: control (n = 12), experimental (n = 8), treatment (n = 5).