Figure 5
From: MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases
Relative expression of miRNA candidates before and after induction of distinct gastrointestinal diseases in mice. The expression level of miR-375 (a–c), miR-30b (d–f), miR-203 (g–i) and miR-132 (j–l) was analyzed in the blood plasma of healthy mice before any intervention (c), compared to the early phase of pancreatitis, or at the late phase of pancreatic cancer progression or bile duct ligation, either on mice treated with vehicle (exp.) or undergoing therapy (treat.). Statistically significant differences of non-parametric data were analyzed by Kruskal–Wallis test and Dunn’s method (e, h, i, l). Parametric data was calculated by one-way ANOVA, followed by Tukey’s multiple comparisons test (b, c, f, k). Parametric data for single comparison was calculated using unpaired t-test (a) and non-parametric data was analyzed by Mann–Whitney test (d, g, j). P < 0.05 was considered to be significant. Pancreatitis: c (n = 11), exp. (n = 10), Pancreatic cancer: c (n = 11), exp. (n = 9), treat. (n = 4); Bile duct ligation: c (n = 12), exp. (n = 8), treat (n = 6).
