Figure 3 | Scientific Reports

Figure 3

From: Deletion of CD38 and supplementation of NAD+ attenuate axon degeneration in a mouse facial nerve axotomy model

Figure 3

Comparison of time-dependent changes of glial cells and peripheral inflammatory cells between WT and CD38 CD38 knockout (KO) mice. (A) Facial nuclei from WT and CD38 KO mice immunostained with glial fibrillary acidic protein (GFAP) (green) and DAPI (blue) at post-operative day (POD) 7. Scale bar, 100 μm. (B,C) Time-dependent changes in the number (B) and the size (C) of GFAP + cells. (D) Facial nuclei from WT and CD38 KO mice immunostained with Iba1 (red) and DAPI (blue) at POD7. Scale bar, 100 μm. (E,F) Time-dependent changes in the number (E) and the size (F) of Iba1 + cells. (G,J) Cross sections of facial nerves from WT and CD38 KO mice immunostained with Iba1 (G) and Gr-1 (J) at POD1, POD3, and POD7. Scale bar, 100 μm. (H,I,K) The number (H) and the size (I) of Iba1-positive cells and the number of Gr-1-positive cells (K). Data are expressed as mean ± SEM. n = 4. *p < 0.05, **p < 0.01, as determined by t-test.

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