Figure 5
Effects of nicotinamide riboside (NR) administration on the facial nuclei and nerves. (A) Nissl staining of neurons in the facial nuclei of WT (NR(−)) and NR-administered WT (NR( +)) mice. The area circled in white is the facial nucleus. (B) The time-dependent changes of the number of surviving cells on the nerve transection side in NR− and NR + mice (n = 4). (C) Facial nerve cross-sections from NR − and NR + mice at POD3 and POD7 immunostained with myelin basic protein (MBP) (green) and β3-tubulin (red). The images on the right are enlarged views of the areas enclosed in white. (D,E) Time-dependent changes in the number of axons (D) and myelin sheaths (E) per mm2 in facial nerve sections. Data are expressed as mean ± SEM. n = 4. *p < 0.05, **p < 0.01, as determined by t-test. Scale bar, 100 μm.
