Figure 6
Comparison of time-dependent changes of glial cells and peripheral inflammatory cells in WT mice with and without nicotinamide riboside (NR) administration. (A) Facial nuclei from WT (NR(−)) and NR-administered WT (NR( +)) mice immunostained with GFAP (green) and DAPI (blue) at POD7. Scale bar, 100 μm. (B,C) Time-dependent changes in the number (B) and the size (C) of GFAP + cells. (D) Facial nuclei of WT and NR mice immunostained with Iba1 (red) and DAPI (blue) at POD7. Scale bar, 100 μm. (E,F) Time-dependent changes in the number (E) and the size (F) of Iba1 + cells. (G,J) Cross-sections of facial nerves from WT and NR mice immunostained with Iba1 (G) and Gr-1 (J) at POD1, POD3, and POD7. (H–K) The numbers (H) and the size (I) of Iba1-positive cells and the number of Gr-1-positive cells (K). Data are expressed as mean ± SEM. n = 4, respectively. *p < 0.05, **p < 0.01, as determined by t-test.
