Figure 6
From: A novel RGB-trichrome staining method for routine histological analysis of musculoskeletal tissues
Staining of osteoid osteomas with the RGB staining method, showing bone formation and resorption. Trabeculae (Tb) show different degrees of calcification, from osteoid (A), to partially calcified (B) and calcified (C) matrix. The surface of the trabeculae show abundant and prominent osteoblasts (Ob in D). Osteoblasts (arrows in D) lay down bone matrix in the scaffold of dense fibrous tissue. Newly formed woven bone (WB, in E,F) is crossed by collagen fiber bundles (CFB), which are evident even after partial calcification (CB in E). Multinucleated osteoclasts (Oc) in the surface of trabeculae of woven bone (G,H) are also observed.
