Figure 8 | Scientific Reports

Figure 8

From: Magnetic separation of peripheral nerve-resident cells underscores key molecular features of human Schwann cells and fibroblasts: an immunochemical and transcriptomics approach

Figure 8

Ligand-receptor profiling in SCs and fibroblasts. (a–b) Distribution of transcripts encoding for soluble polypeptide growth factors and their cognate plasma membrane receptors. The figure displays selected candidate mediators of SC-fibroblast communication and nerve regeneration as initially highlighted by the output of GO biological processes and Reactome. The most relevant variants/isoforms (> 5 RCPM in SCs or fibroblasts) from each gene family are listed. (c–f) Distribution of transcripts involved in axon guidance and growth. GSEA enrichment plot as per Homo sapiens Reactome ID 44245 (c) showing non-overlapping still nearly equal enrichment of axon guidance genes in SCs and fibroblasts. Whereas adhesion molecules (CHL1, NCAM1, NRCAM, L1CAM), ECM molecules (COL9A3, COL9A2), and components of plexin signaling (PLXNB3, SEMA6A, SEMA4D) were highlighted in SCs (d), integrin receptors (ITGA10), and components of the ephrin (EPHB6, EPHA7, EPHB2) and netrin signaling pathways (NTN4, UNC5C, ABLIM1) were highlighted in fibroblasts (e–f). Ligand-receptor pairing was possible for all of the identified classes of ligands (i.e., secreted and membrane-bound ligands) with the exception of VEGF family members (a) whose receptors are expected to be present in vascular cells.

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