Table 1 MACS of human SC cultures affected by different degrees of fibroblast growth.

Fibroblasts/SCs	Marker	Positive cells (%)			p value	Recovery (%)	Column type
Fibroblasts/SCs	Marker	Total	Eluted	Retained	p value	Recovery (%)	Column type
High	p75^NGFR	20.2 ± 3.9	0.0 ± 0.0	72.1 ± 3.2	< 0.001	76	MS
	S100β	15.5 ± 2.3	0.0 ± 0.0	76.2 ± 4.0	< 0.001	76	MS
	p75^NGFR	27.0 ± 4.6	0.0 ± 0.0	73.7 ± 4.9	< 0.001	75	MS
	S100β	18.1 ± 4.2	0.0 ± 0.0	74.2 ± 5.2	< 0.001	75	MS
	p75^NGFR	70.8 ± 6.7	0.9 ± 0.4	97.6 ± 1.2	< 0.001	51	MS
	S100β	72.6 ± 6.0	0.9 ± 0.4	97.8 ± 1.3	< 0.001	51	MS
Moderate	p75^NGFR	75.1 ± 5.9	1.2 ± 0.7	98.1 ± 0.5	< 0.001	n.d	MS
	S100β	75.3 ± 7.0	1.2 ± 0.7	98.0 ± 0.7	< 0.001	n.d	MS
	p75^NGFR	82.6 ± 2.3	23.6 ± 2.5	99.5 ± 0.2	< 0.001	100	Large cell
	S100β	78.1 ± 4.0	23.5 ± 4.1	99.6 ± 0.2	< 0.001	100	Large cell
	p75^NGFR	88.1 ± 3.6	11.5 ± 4.4	98.7 ± 0.5	< 0.001	54	MS
	S100β	87.5 ± 2.1	11.2 ± 4.1	98.7 ± 0.5	< 0.001	54	MS
Low	p75^NGFR	88.9 ± 3.3	3.3 ± 1.7	95.6 ± 0.6	< 0.01	n.d	MS
	S100β	87.8 ± 2.6	3.3 ± 1.7	95.5 ± 0.5	< 0.01	n.d	MS
	p75^NGFR	93.1 ± 3.1	33.9 ± 5.0	98.8 ± 0.9	< 0.05	91	Large cell
	S100β	90.9 ± 1.9	33.3 ± 4.0	99.9 ± 0.2	< 0.001	91	Large cell

Cellular material from 8 different cultures were subjected to purification via p75^NGFR immunolabeling and MACS using two types of columns, as indicated. Experimental conditions were identical to those described in Figs. 4. Passage and donor information for each culture was the following (from top to bottom): passage-2 (17 years old, male); passage-3 (17 years old, male); passage-2 (66 year old, male), passage-2 (18 year old, male); passage-1 (60 year old, male); passage-3 (10 year old, female); passage-3 (60 year old, male); passage-1 (66 year old, male). Quantification of S100β and p75^NGFR was carried out by microscopic analysis of serial images obtained by automated fluorescence microscopy. The p-value results from the comparison between samples from total and retained cells (T-test). The viability was determined by Trypan blue exclusion assays using cells in suspension collected immediately after separation. Non-determined measurements are indicated as n.d. Some variability was observed in the percentage of SC enrichment in the retained fraction, as determined primarily by the initial proportion of SCs versus fibroblasts. The recovery of cells (i.e. the ratio between the pre- and post-sorting cell numbers) was variable using MS columns but consistently high using Large Cell columns, which are designed for the separation of cells of a larger diameter.

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