Figure 2

Evaluation of human, bacterial and fungal DNA by extraction protocol. Boxplot of Ct values for β-actin gene (A), 16S rRNA (B) and 18S rRNA gene (C) obtained with the five extraction protocols. Solid black lines indicate medians, and the lower and upper bounds of the box represent the 25 and 75% quartiles. Outliers, defined as falling outside the 10% and 90% quartiles, are indicated with black circles. Significant differences based on the Kruskal–Wallis test are specified in the figure.