Figure 5
Validation of the pcPCR-HRM technique to genotype mdx mouse. (a) Primer sequences and expected product sizes from Shin et al. 16. Bold letters represent allele-specific nucleotides at the position of the mdx mutation. Non-specific synthetic tails are underlined. (b) Genotypes of three wild-type, three heterozygous and three mdx mice resolved by 3% agarose electrophoresis after pcPCR. (c) HRM difference melt plots from 9 mice (same as in b) performed in triplicate with the mdx cluster as a baseline. Wild-type (blue), heterozygous (red), and mdx (green) clusters are identified on the melt plot. (d) Tm values from pcPCR products (same as in c): wild-type (blue), heterozygous (red) and, mdx (green) genotypes. Data are expressed as mean ± SD. One-way ANOVA with Tukey’s multiple comparisons test was used for statistical analysis.
