Figure 5 | Scientific Reports

Figure 5

From: A systematic identification of anti-inflammatory active components derived from Mu Dan Pi and their applications in inflammatory bowel disease

Figure 5

Inhibitory effects of metabolites from PGG and MDP on NF-kB and IRF reporters. THP1-Dual cells were treated with conditioned medium obtained from supernatants of cells treated with 50 uM PGG or 0.5% WE for different time periods (0, 3, 6, or 24 h), then stimulated with Pam3CSK4 or poly(I:C) for 24 h and NF-κB (A, D) and IRF (B, E) reporter activities were measured. (C, F) The MTS assay determined the viability of THP1-Dual cells subjected to metabolite and Pam3CSK4 stimulation. Control cells labeled with Pam3CSK4 or poly(I:C) underwent stimulation only. Data are presented as the mean ± S.D (n = 3) and analyzed using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001.

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