Figure 2
From: Gfi1 upregulates c-Myc expression and promotes c-Myc-driven cell proliferation
Gfi1 reduces the polyubiquitination and increases the stability of c-Myc protein. (A) BaF/MycER/Gfi1 (upper panels) and BaF/Gfi1 (lower panels) cells were untreated (Ctr) or treated with Dox for 24 h, followed by treatment with cycloheximide (CHX; 30 μg/ml) for different times. The blots were cropped as indicated by the vertical lines. (B) Hela cells were transfected as indicated, and then treated with MG132 at different concentrations for 6 h prior to preparation of whole cell extracts. (C) Hela cells were transfected as indicated and treated with MG132 for 6 h. Whole cell extracts (WCE) were subjected to immunoprecipitation using the HA antibody under the denaturing condition. c-Myc expression and polyubiquitination were examined by Western blot analysis. Full-length blots are presented in Supplementary Fig. 7.
